Introduction​

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Electrochemical Impedance Spectroscopy (EIS) is a well-established scientific method used to characterize the electrical properties of organic and biological materials by applying a weak electrical signal at a specific frequency and measuring the resulting electrical response. EIS measurements provide information about electrical properties including impedance, conductivity, resistance, and charge storage within biological systems (Naranjo-Hernández et al., 2019; Brantlov et al., 2025). Characterization of the electrical properties of biological samples has been used to investigate cell membrane function and distinguish differences between biological tissues. Previous research has demonstrated that EIS can characterize the electrical properties of human buccal (inner cheek) cells and provide information regarding the interaction between the cell membrane and its surrounding environment (Yang et al., 2011). The absorption and

interaction of electromagnetic energy with biological systems is highly dependent upon the electrical impedance characteristics of the target tissue and the frequency of the applied signal (Hossain, 2021). Because EIS allows impedance measurements to be obtained at specific frequencies, it provides a useful method for evaluating changes in

cellular electrical properties following an experimental exposure. In the present study, EIS was used to measure the electrical properties of living human buccal cells before and during extended exposure to a large TLS Pyramid. Measurements were obtained at 1.39 kHz, a frequency previously identified by the Quantum Biology Research Lab as a

resonant frequency of the buccal-cell preparation used in its experiments. The study specifically evaluated changes in cellular impedance. Because impedance and conductivity are inversely related, a decrease in impedance corresponds to an increase in electrical conductivity. The results demonstrate that exposure to the TLS Pyramid produced measurable decreases in cellular impedance, corresponding to increased conductivity. Two different exposure protocols were evaluated to determine whether the participant's activity and position relative to the Pyramid influenced the magnitude or duration of the measured bioelectrical response.

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Scientific Basis

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Electrochemical Impedance Spectroscopy measures how electrical energy moves through and interacts with biological material. In biological systems, these measurements are influenced by characteristics including cell membrane properties, extracellular fluid, intracellular composition, and the movement and storage of electrical charge. Electrical

conductivity describes the ability of a material or biological system to conduct electrical current. Conductivity and impedance are inversely related: when impedance decreases, conductivity increases. Electrical conductivity is relevant to biological function because living systems depend upon electrical activity for cellular communication and numerous

physiological processes. Previous experimental research has also demonstrated that electrical stimulation can influence cellular signaling pathways associated with inflammation and oxidative stress (Jeong et al., 2013; Tanaka et al., 2016; Hsieh et al., 2019). Bioimpedance techniques have therefore become useful research tools for evaluating changes in the electrical characteristics of biological systems (Naranjo- Hernández et al., 2019; Brantlov et al., 2025). The Quantum Biology Research Lab has modified the standard EIS methodology through changes to electrode material and

geometry. Measurements are obtained at frequencies selected for the biological target being evaluated. In the present experiment, measurements were performed at 1.39 kHz. By comparing impedance measurements obtained before and during exposure to the TLS Pyramid, the experiment was designed to determine whether Pyramid exposure produces

measurable changes in cellular electrical conductivity and whether the magnitude of those changes differs depending upon exposure conditions.

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What This Study Demonstrates

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This study was designed to evaluate the bioelectrical effects produced during extended exposure to a large TLS Pyramid. Two separate protocols were examined. In the first, referred to as the moving protocol, the participant remained within approximately three to four feet of the TLS Pyramid while continuing to move throughout the room during the exposure period. In the second, referred to as the stationary protocol, the participant remained seated approximately three feet in front of the Pyramid for an extended period and subsequently slept approximately three feet from the Pyramid while it remained operating. Both protocols produced a similar initial response. Cellular impedance progressively decreased during the first several hours of exposure, reaching the largest response at approximately four hours. The maximum change was approximately 50%, corresponding to an approximately 50% increase in conductivity relative to baseline as

reported in the original experiment. However, an important difference emerged as exposure continued. During the moving protocol, the measured response progressively returned toward baseline. During the stationary protocol, a substantial conductivity response remained detectable after extended exposure. These findings indicate that the

TLS Pyramid produces measurable changes in cellular electrical conductivity and suggest that remaining stationary and near the Pyramid may produce a more sustained bioelectrical response than moving throughout the surrounding environment.

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Methods

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The bioelectrical effects of the large TLS Pyramid were evaluated using the modified Electrochemical Impedance Spectroscopy methodology described above. Living human buccal cells were collected from the inner cheek and transferred into water to create a cell suspension. Two electrodes were immersed in the resulting sample and connected to the impedance measurement system. Three sequential samples were measured during each collection period. Each sample was measured in triplicate and the average value was calculated. According to the original experimental observations, impedance changed by approximately 1–5% during the approximately two-minute measurement procedure, indicating relative stability of the preparation over the measurement interval. Baseline impedance measurements varied between approximately 35 and 50 kΩ on different experimental days. Because baseline values differed between testing sessions, all subsequent measurements were calculated as percent change relative to the baseline measurement obtained on the corresponding experimental day. A separate control experiment was also performed under normal ambient environmental conditions. During this control period, the participant did not use a cell phone or computer for five hours. Buccal-cell impedance changed by approximately 5%, which was used in the original

experiment as an estimate of normal experimental variability.

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Moving Protocol

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Following baseline measurement, the participant entered the room containing the TLS Pyramid and remained approximately three to four feet from the device while moving throughout the area during the day. The participant remained under these conditions for approximately 13 hours. During the experimental period, he did not use a computer or cell phone in order to minimize possible external electromagnetic influences. The Pyramid was subsequently turned off for approximately nine hours while the participant slept, after which an additional measurement was obtained the following morning.

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Stationary Protocol

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During the stationary protocol, the participant remained seated approximately three feet in front of the TLS Pyramid for eight hours. He did not use a computer or cell phone during this period. A television approximately ten feet away was used during part of the exposure. Following the daytime exposure period, the TLS Pyramid remained operating

for an additional seven hours while the participant slept approximately three feet from the Pyramid. Additional measurements were obtained during the extended exposure period. All results are presented as percent change relative to the corresponding baseline measurement.

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Results and Discussion

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Moving Exposure

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Figure 1 illustrates the change in cellular impedance observed during the moving protocol.

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Exposure to the TLS Pyramid produced a progressive decrease in impedance during the initial hours of treatment, corresponding to increased cellular conductivity. The strongest response occurred approximately four hours after exposure began, when the measured change reached approximately 50% relative to baseline. Following the four-hour peak, the magnitude of the response gradually decreased. By approximately eight hours, impedance had returned close to baseline and subsequently showed a small change in the opposite direction. Because the control experiment demonstrated approximately 5% variability under ambient conditions, the small opposite-direction response observed later i n the moving protocol was close to the estimated range of experimental variability. The moving protocol therefore demonstrates a rapid increase in cellular conductivity during the first several hours of TLS Pyramid exposure, with the largest response occurring at approximately four hours and progressively diminishing thereafter.

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Stationary Exposure

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Figure 2 illustrates the response observed when the participant remained stationary and in close proximity to the TLS Pyramid.

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The initial response was similar to that observed during the moving protocol. Impedance progressively decreased during the first several hours, again reaching a maximum change of approximately 50% at four hours. However, the later response differed considerably. Rather than returning toward baseline as rapidly as observed during the moving protocol, a substantial change remained detectable during continued stationary exposure. According to the original measurements, an approximately 30% response remained after extended exposure, including measurements obtained after approximately eight and thirteen hours. The similarity between the initial responses of the two protocols is

notable. Both produced their largest measured effect at approximately four hours, and both reached a magnitude of approximately 50%. The primary difference was therefore not the magnitude of the initial peak response, but rather how long the response remained elevated.

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Effect of Exposure Conditions

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Taken together, the two protocols suggest that the participant's position and activity during TLS Pyramid exposure may influence the duration of the measured bioelectrical response. Both moving and stationary exposure produced a similar initial decrease in impedance and corresponding increase in conductivity. However, during the moving protocol, the effect progressively returned toward baseline, whereas the stationary protocol produced a more sustained response during extended exposure. These findings suggest that remaining stationary and within close proximity to the TLS Pyramid may allow the measurable conductivity response to persist for a longer period of time. This observation may also have practical implications for nighttime exposure. Because individuals naturally remain relatively stationary while sleeping, positioning the Pyramid near the sleeping environment could provide a practical method of maintaining

prolonged stationary exposure. However, this experiment evaluated cellular electrical measurements in a single participant. Additional studies involving larger participant groups would be necessary to determine whether the difference between moving and stationary

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Conclusion

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This study demonstrates that extended exposure to a large TLS Pyramid produces measurable changes in cellular electrical properties as measured using Electrochemical Impedance Spectroscopy. Under both moving and stationary exposure conditions, cellular impedance decreased during the initial hours of exposure, corresponding to an increase in electrical conductivity. The strongest response occurred approximately four hours after exposure began, with both experimental protocols producing an approximately 50% change relative to baseline. The primary difference between the protocols was observed during continued exposure. During the moving protocol, the bioelectrical response progressively returned toward baseline. In contrast, during the stationary protocol, an approximately 30% response remained detectable during later measurements, indicating a more sustained change in cellular conductivity. These

findings provide quantitative evidence that exposure to the TLS Pyramid produces measurable changes in cellular bioelectrical activity and further indicate that exposure conditions may influence the duration of the response. The results suggest that remaining stationary and in close proximity to the Pyramid may produce a more sustained bioelectrical effect than moving throughout the surrounding environment. Because sleeping naturally provides an extended period of stationary exposure, nighttime use near the Pyramid may represent a practical approach for prolonged exposure based on the response observed in this experiment. Taken together, the findings demonstrate a

strong and time-dependent cellular conductivity response during TLS Pyramid exposure, with a maximum effect observed after approximately four hours and a more sustained response observed during stationary exposure. Because this experiment was performed in a single participant, further controlled studies involving larger populations are needed to determine the reproducibility of these findings and the biological significance of the observed conductivity changes.