Introduction​

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This study evaluates the bioelectrical effects produced by two TLS Cube Minis positioned opposite one another using Electrochemical Impedance Spectroscopy (EIS). EIS is a well-established scientific method used to characterize electrical properties of biological systems, including impedance and conductivity, and has been used to investigate the electrical behavior of living cells and cell membranes (Yang et al., 2011; Hossain, 2021; Brantlov et al., 2025). Previous testing conducted by the Quantum Biology Research Lab demonstrated that exposure to a single TLS Cube Mini produced measurable changes in the electrical properties of living human buccal (inner cheek) cells. The single-device configuration was characterized primarily by decreased impedance, expressed as increased conductivity. The present experiment evaluated a different configuration in which a subject was positioned between two opposing TLS Cube Minis. The objective was to determine whether this configuration would produce a measurable cellular bioelectrical response and to characterize that response through the real and imaginary components of impedance.

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Scientific Basis

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Electrochemical Impedance Spectroscopy measures the response of biological materials to a small applied alternating electrical signal. When applied to living cells, EIS provides quantitative information about electrical characteristics associated with cellular membranes and their surrounding environment (Yang et al., 2011; Hossain, 2021; Naranjo-Hernández et al., 2019). Electrical conductivity contains both real and imaginary components. The real component represents the resistive component of the measured electrical response, while the imaginary component reflects its reactive or capacitive characteristics. Evaluating both components provides a more complete characterization of changes in the electrical properties of a biological sample. Bioconductivity measurements have been investigated in relation to cellular and tissue characteristics including cell-membrane properties, fluid distribution, and other physiological variables (Yamada et al., 2022; Catapano et al., 2023; Kim et al., 2025). Accordingly, changes in real and imaginary conductivity provide measurable indicators that the electrical characteristics of the biological sample have changed. The present study uses these parameters to characterize the response observed following exposure to two opposing TLS Cube Minis.

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What This Study Demonstrates

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This study was designed to evaluate the bioelectrical response associated with positioning a subject between two opposing TLS Cube Minis. Following one hour of exposure, a substantial increase in cellular conductivity was observed. The response occurred predominantly in the real component of conductivity, while a smaller increase was observed in imaginary conductivity. The source report describes an approximately 92% increase in conductivity after one hour, demonstrating a large measurable change relative to baseline. Importantly, the response pattern observed with two opposing Cube Minis differed from that previously observed with a single Cube Mini. Whereas the single Cube Mini produced a response characterized primarily by increased conductivity, the opposing configuration produced a response characterized primarily by a more significant increased conductivity. These findings demonstrate that the configuration of the TLS Cube Minis was associated with a more significant measurable bioelectrical response pattern under the conditions evaluated.

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Methods

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The bioelectrical effects of two opposing TLS Cube Minis were evaluated using Electrochemical Impedance Spectroscopy. Living human buccal cells were collected from the inner cheek and transferred into water for analysis. Two electrodes connected to an impedance spectrophotometer were immersed in the resulting cell suspension, allowing changes in cellular electrical properties to be measured. Baseline measurements were obtained before exposure. The subject was then positioned between two TLS Cube Minis arranged opposite one another for a one-hour exposure period. Following exposure, additional buccal-cell samples were collected and analyzed using the same EIS methodology. Each collected sample was measured in triplicate, and the average measurement was used for analysis. Changes were calculated relative to the baseline measurement obtained before exposure. Both real and imaginary conductivity were evaluated to characterize the nature and magnitude of the measured bioelectrical response.

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Results and Discussion

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Following one hour of exposure between two opposing TLS Cube Minis, a substantial increase in cellular conductivity was observed as seen in Figure 1. The source report describes an approximately 92% increase in conductivity, indicating a strong measurable bioelectrical response following the one-hour exposure period. Analysis of the individual conductivity components showed that the response was concentrated primarily in real conductivity, while imaginary conductivity increased to a considerably smaller degree.

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The difference between the real and imaginary components provides additional information about the nature of the measured response. Rather than both components changing by the same magnitude, the real component accounted for most of the observed increase. Another notable finding is that the response differed from that observed during exposure to a single TLS Cube Mini. In the single-device experiment, the principal response was expressed as a less significant increase in conductivity resulting from decreased measured impedance. Whereas, positioning the subject between two opposing Cube Minis produced a more significant increase in conductivity. This difference indicates that device configuration influenced the magnitude of the measured EIS response.

Taken together, the results provide quantitative evidence that one hour of exposure between two opposing TLS Cube Minis was associated with a substantial and measurable increase in the electrical conductivity of living human buccal cells.

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Conclusion

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The results of this study demonstrate that positioning a subject between two opposing TLS Cube Minis was associated with a strong measurable bioelectrical response after one hour of exposure. The source report describes an approximately 92% increase in cellular conductivity, with the response occurring predominantly within the real component of conductivity and a smaller increase occurring within the imaginary component. Importantly, the opposing configuration produced a more significant EIS response from that previously observed with a single Cube Mini. Whereas the single-device configuration was characterized primarily by a smaller increase in conductivity, the opposing configuration was characterized by a more significant increase in conductivity. These findings indicate that the physical configuration of the TLS Cube Minis was associated with differences in the measured cellular electrical response. Taken together, the results provide quantitative evidence that exposure between two opposing TLS Cube Minis produces a substantial difference in cellular bioelectrical properties within a one-hour exposure period.