Introduction

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This study evaluates the effect of the TLS Supercharging Process on a TLS Medallion by measuring changes in bioelectrical activity using Electrochemical Impedance Spectroscopy (EIS). EIS is a well-established scientific method used to assess electrical properties such as impedance and conductivity at the cellular and molecular level and has been widely used to evaluate changes in biological systems (Yang, 2011; Becchi, 2005). In this study, EIS was used to measure how energy emitted from a TLS Supercharged Medallion interacts with living human cheek cells (buccal cells). The objective was to compare the measurable bioelectrical effects of an identical Tiger Eye Medallion before and after undergoing the proprietary TLS Supercharging Process. By comparing a TLS Supercharged Medallion to an identical Uncharged Medallion used as a control, this study provides quantitative evidence regarding the impact of the TLS Supercharging Process on cellular bioelectrical activity and the duration of its effects.

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Scientific Basis

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Electrochemical Impedance Spectroscopy (EIS) measures how electrical energy moves through, interacts with, and is stored within biological systems. These measurements provide information about the electrical properties of cell membranes and the body's overall bioelectrical state. Changes in EIS measurements are commonly reflected as changes in electrical impedance or electrical conductivity. Increased impedance is associated with the accumulation and storage of electrical charge at the cellular membrane, while increased conductivity reflects the more efficient movement of electrical energy through biological tissues. Both responses have been linked in scientific literature to important physiological processes including cellular integrity, fluid balance, cellular communication, and energy transfer (Yamada, 2022; Kim, 2025; Jeong, 2013; Hsieh, 2019). Because biological systems rely on electrical activity to regulate cellular function, measurable changes in impedance or conductivity indicate that an external influence is affecting the body's bioelectrical environment. By comparing an UnchargedMedallion to an identical TLS Supercharged Medallion, this study evaluates whether the TLS Supercharging Process produces measurable differences in cellular bioelectrical

activity beyond those attributable to the medallion material alone. What This Study Demonstrates This study was designed to evaluate the measurable bioelectrical effects of the TLS Supercharging Process by comparing a TLS Supercharged Medallion to an identical Uncharged Medallion used as a control. Measurements were obtained using Electrochemical Impedance Spectroscopy (EIS) following exposure to each medallion under the same testing conditions.

 

The results demonstrate that the Uncharged Medallion produces only minimal changes in bioelectrical activity, while the TLS Supercharged Medallion produces substantially larger and more measurable effects. After one hour of wear, the TLS Supercharged Medallion produced a 175% increase in impedance compared to 6.8% for the Uncharged Medallion. After three hours of wear, the TLS Supercharged Medallion produced a 382% increase in impedance compared to 8.6% for the Uncharged Medallion. The study further demonstrates that the bioelectrical effects of the TLS Supercharged Medallion persist beyond the active wear period. Elevated impedance levels remained detectable for up to twenty-four hours after removal, indicating that the measured response continues well after exposure has ended. Together, these findings provide quantitative evidence that the TLS Supercharging Process significantly amplifies the measurable bioelectrical effects of the medallion and produces rapid, strong, and sustained changes in cellular bioelectrical activity.

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Methods

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The bioelectrical effects of a TLS Supercharged Medallion were evaluated using Electrochemical Impedance Spectroscopy (EIS). Measurements were performed on living human buccal (inner cheek) cells collected before and after exposure to the medallion. A TLS Supercharged Tiger Eye Medallion was worn continuously for one hour and three

hours under separate test conditions. An identical Uncharged Tiger Eye Medallion was used as a control and evaluated using the same protocol. Baseline measurements were obtained prior to each treatment, and impedance measurements were collected following exposure to determine changes in cellular bioelectrical activity. To evaluate the persistence of the effect, additional measurements were obtained following removal of the TLS Supercharged Medallion over a twenty-four-hour period. Each sample was measured in triplicate and the average value was used for analysis. All results are reported as percent change relative to baseline measurements obtained prior to treatment.

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Results and Discussion

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Figure 1 compares the bioelectrical effects of a TLS Supercharged Medallion to an identical Uncharged Medallion during active wear. After one hour, the UnchargedMedallion produced only a modest 6.8% increase in impedance, while the TLS Supercharged Medallion produced a 175% increase, representing a response approximately 25.7 times greater. These findings demonstrate a clear and measurable difference between the Uncharged Medallion and the TLS Supercharged Medallion within the first hour of exposure (Figure 1).

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After three hours of wear, the Uncharged Medallion produced an 8.6% increase in impedance, while the TLS Supercharged Medallion produced a 382% increase - a response approximately 44.4 times greater (Figure 1). This was the largest bioelectrical effect observed in the study and demonstrates that the TLS Supercharging Process significantly amplifies the medallion’s measurable bioelectrical impact. Figure 1 also shows that the response increases with continued wear, reaching its peak after three hours of exposure.

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Figure 2 illustrates the persistence of the bioelectrical response following removal of the TLS Supercharged Medallion. Although the effect gradually declined over time, impedance remained elevated by approximately 130% after twenty-four hours, indicating that the response continued well beyond the active wear period. Based on the observed response curve, the effects of a three-hour exposure may persist for up to forty-eight hours after removal.

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Taken together, these findings demonstrate that the TLS Supercharged Medallion produces rapid, strong, and sustained bioelectrical effects that greatly exceed those observed with an identical Uncharged Medallion. The results provide quantitative evidence that the TLS Supercharging Process significantly enhances the measurable bioelectrical impact of the medallion and produces effects that continue long after exposure has ended.

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Real and Imaginary Energies

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EIS allows for analysis of both real impedance (energy dissipation) and imaginary impedance (energy storage). The data in Figure 3 reflects the comparison of both real and imaginary energies of water treated with the TLS Medallion for 24 hours. To obtain a complete characterization of the TLS Medallion, both need to be measured. Imaginary impedance is reported in the scientific literature and is known to give different information than real impedance about the properties of a given medallion. In nearly all cases, real and imaginary values parallel each other. This means that in response to a given treatment, both generally show an increase simultaneously or a decrease simultaneously. However, the data in this study indicates the opposite phenomenon: water responded to the energy of the medallion by increasing the real impedance and decreasing the imaginary impedance (Figure 3).

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Previous observations from the Quantum Biology Research Lab indicate that when real and imaginary responses move in different directions, the system may be exhibiting non- classical or quantum-like behavior. The results observed here are consistent with that pattern.

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Conclusion

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Using Electrochemical Impedance Spectroscopy (EIS), this study demonstrates that an Uncharged Medallion produces only minimal bioelectric change, while a TLS Medallion which undergoes the proprietary TLS Supercharging process generates rapid, large, and measurable effects. These effects occur within hours rather than days, are sustained overtime, and can persist for up to 48 hours after exposure terminates. The data also shows that this energy transfer is detectable in both biological systems and water, reinforcing the consistency of the findings across different mediums. Additionally, the observed impedance patterns suggest the presence of non-classical energetic behavior. Interpretation of the data suggests that the energy emitted by the medallion is quantum in nature and not electromagnetic. Overall, these results confirm that the proprietary TLS Supercharging Process significantly amplifies the measurable bioelectric impact of a medallion, producing faster, stronger, and more sustained effects compared to its uncharged state.

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